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KMID : 0379520060220040349
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한국독성학회지
2006 Volume.22 No. 4 p.349 ~ p.356
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The Effects of Diallyl Disulfide on Antimetastatic Potential of B16-F10 Murine Melanoma Cells
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Kang Mi-Kyung
Jun Hye-Seung
Yum Young-Na
Hwang Myung-Sil
Park Mi-Sun
Kim Ok-Hee
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Abstract
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Diallyl disulfide (DADS), an oil-soluble organosulfur compound in garlic has been reported to suppress tumor growth and to induce apoptosis in cancer. In the present study, we investigated the effects of DADS on pulmonary metastasis of B16-F10 murine melanoma cells. DADS (i.p. 40 mg/kg) significantly (p<0.05) reduced the number of pulmonary metastatic nodules (48%) in experimental pulmonary metastasis assay. We also found that DADS inhibited adhesion, invasion and migration of B16-F10 melanoma cells in a dose-dependent manner. To study the antimetastatic potential of DADS, we performed the effects of DADS on matrix metalloproteinase activity. DADS significantly inhibited the expression of matrix metalloproteinase-2 activity in B16-F10 cells by gelatin zymography. These results suggest that DADS prevent metastasis in part through suppression of migration of B16-F10 melanoma cells by Inhibiting matrix metalloproteirase-2 responsible for degradation of extracellar matrix.
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KEYWORD
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Diallyl disulfide, B16F10 melamona cells, Experimental pulmonary metastasis assay, Invasion, Matrix metalloproteinase-2
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